The purpose of the study was to know the physical and physiological profiles of different levels of male Handball players. Ninety male Handball players from different universities those who participate in intercollegiate, interuniversity and inter zonal tournament. Standardized test was applied to measure physical variable i.e. standing height, sitting height, leg length, upper arm length, forearm length, hand length, body composition and physiological variable i.e. resting pulse rate, resting B.P., vital capacity, resting respiratory rate, maximum breath holding time, air flow rate and cardio-vascular efficiency. Analysis of variance and LSD Post hoc test was applied to assess the significance difference among the group means. The level of significance was set at 0.05. The result of study showed that the entire physical variable of Handball players of different levels of achievement except hand length was different significantly. But, no significance difference in physiological variables of Handball players of different levels of achievement was found.